Global identification and functional characterization of Z-DNA in rice.

ABSTRACT: Z-DNA is a left-handed double helix form of DNA that is believed to be involved in various DNA transactions. However, comprehensive investigations aimed at global profiling of Z-DNA landscapes are still missing in both humans and plants. We here report the development of two techniques: anti-Z-DNA antibody-based immunoprecipitation followed by sequencing (ZIP-seq), and cleavage under targets and tagmentation (CUT&TAG) for characterizing Z-DNA in nipponbare rice (Oryza sativa L., Japonica). We found that Z-DNA-IP⁺ (Z-DNA recognized by the antibody) exhibits distinct genomic features as compared to Z-DNA-IP^- (Z-DNA not recognized by the antibody). The concomitant presence of G-quadruplexes (G4s) and i-motifs (iMs) may promote Z-DNA formation. DNA modifications such as D

SUBMITTER: He Z 

PROVIDER: S-EPMC11933839 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature