Metabolite-Responsive Control of Transcription by Phase Separation-Based Synthetic Organelles.

ABSTRACT: Living natural materials have remarkable sensing abilities that translate external cues into functional changes of the material. The reconstruction of such sensing materials in bottom-up synthetic biology provides the opportunity to develop synthetic materials with life-like sensing and adaptation ability. Key to such functions are material modules that translate specific input signals into a biomolecular response. Here, we engineer a synthetic organelle based on liquid-liquid phase separation that translates a metabolic signal into the regulation of gene transcription. To this aim, we engineer the pyruvate-dependent repressor PdhR to undergo liquid-liquid phase separation in vitro by fusion to intrinsically disordered regions. We demonstrate that the resulting coacervates bind DNA

SUBMITTER: Jerez-Longres C 

PROVIDER: S-EPMC11934134 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature