Toward Antibody Production in Genome-Minimized <i>Bacillus subtilis</i> Strains.

ABSTRACT: Bacillus subtilis is a bacterial cell factory with outstanding protein secretion capabilities that has been deployed as a workhorse for the production of industrial enzymes for more than a century. Nevertheless, the production of other proteins with B. subtilis, such as antibody formats, has thus far been challenging due to specific requirements that relate to correct protein folding and disulfide bond formation upon export from the cytoplasm. In the present study, we explored the possibility of producing functional antibody formats, such as scFvs and scFabs, using the genome-reduced Midi- and MiniBacillus strain lineage. The applied workflow included selection of optimal chassis strains, appropriate expression vectors, signal peptides, growth media, and analyti

SUBMITTER: Schilling T 

PROVIDER: S-EPMC11934139 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature