Thylakoid Targeting Improves Stability of a Cytochrome P450 in the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803.

ABSTRACT: Plants produce a large array of natural products of biotechnological interest. In many cases, these compounds are naturally produced at low titers and involve complex biosynthetic pathways, which often include cytochrome P450 enzymes. P450s are known to be difficult to express in traditional heterotrophic chassis. However, cyanobacteria have shown promise as a sustainable alternative for the heterologous expression of P450s and light-driven product biosynthesis. In this study, we explore strategies for improving plant P450 stability and membrane insertion in cyanobacteria. The widely used model cyanobacterium Synechocystis sp. PCC 6803 was chosen as the host, and the well-studied P450 CYP79A1 from the dhurrin pathway of Sorghum bicolor was chosen as the model enzyme. Combinat

SUBMITTER: Hanamghar SS 

PROVIDER: S-EPMC11934225 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature