Metronidazole response profiles of Gardnerella species are congruent with phylogenetic and comparative genomic analyses.

ABSTRACT:

Background

Bacterial vaginosis (BV) affects 20-50% of reproductive-age female patients annually, arising when opportunistic pathogens outcompete healthy vaginal flora. Many patients fail to resolve symptoms with a course of metronidazole, the current first-line treatment for BV. Our study was designed to identify genomic variation associated with metronidazole resistance among strains of Gardnerella vaginalis spp. (GV), a genus of biogenic-amine-producing bacteria closely associated with BV pathogenesis, for the development of a companion molecular diagnostic.

Methods

Whole-genome sequencing and comparative genomic metrics, including average nucleotide identity and GC content, were performed on a diverse set of 129 GV genomes to generate data for detailed taxonomic analyses.

SUBMITTER: Innamorati KA 

PROVIDER: S-EPMC11934483 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature