A novel rat model of lumbar disc herniation induced by puncture: accurate positioning and controllable degree of herniation.

ABSTRACT:

Background

Lumbar disc herniation (LDH) is the serious stage of intervertebral disc degeneration (IDD), and the location and degree of intervertebral disc herniation are closely related to clinical symptoms and signs. However, there is currently no low-cost, high-benefit animal model to support in vivo research on LDH.

Method

Expose the rat's lumbar 5/6 intervertebral disc through the space between the psoas major and erector spine muscles, and then use different lengths of puncture needles to control the degree of herniation and different puncture angles to push the nucleus pulposus tissue backwards to the different position. Observe the protrusion of intervertebral discs through MRI. Von Frey mechanical pain test and BBB score were used to evaluate the behavior of LDH rats

SUBMITTER: Yang M 

PROVIDER: S-EPMC11934670 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature