Bone marrow mesenchymal stem cell exosomes improve fracture union via remodeling metabolism in nonunion rat model.

ABSTRACT:

Background

Nonunion of fractures is a major unsolved problem in clinical treatment and prognosis of orthopedics. Bone marrow mesenchymal stem cell (BMSC) exosomes have been proven to be involved in mediating tissue and bone regeneration in a variety of diseases. However, the role of BMSC exosomes in fracture nonunion is unclear.

Methods

BMSC exosomes were injected into a rat model of nonunion fracture, and the fracture-healing site was detected by micro-CT and the serum metabolites were analyzed by LC-MS/MS.

Results

The results showed that the exosomes could be successfully isolated from rat BMSCs cultured in an exosome-free medium. Compared with the model group, the fracture site of the exosome-treated rats were healing obviously. Compared with the PBS group, there w

SUBMITTER: Li C 

PROVIDER: S-EPMC11934688 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature