Pushing the Limits of Lateral Flow Immunoassay by Digital SERS for the Ultralow Detection of SARS-CoV-2 Virus.

ABSTRACT: Lateral flow immunoassays (LFIAs) are easy-to-use antigen tests that provide different signal readouts, with colorimetric readouts being the most commonly used. However, these analytical devices have relatively low sensitivity and produce semiquantitative results, limiting their diagnostic applications. Herein, we address these challenges by implementing a digital surface-enhanced Raman spectroscopy (SERS)-based LFIA for the accurate and ultrasensitive quantitative detection of SARS-CoV-2 nucleocapsid (N) protein. Compared with average SERS intensity measurements, the digital approach allowed to overcome fluctuations in Raman scattering signals, thereby increasing the analytical sensitivity of the assay. Our method exhibited a quantification range of the viral protein in nasal swabs from 0

SUBMITTER: Gonzalez-Cabaleiro L 

PROVIDER: S-EPMC11935054 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature