Modeling the Depth Resolution of Translucent Layers in Confocal Microscopy.

ABSTRACT: Confocal microscopy is an established technique with manifold applications that offers the capability to perform nondestructive through-plane imaging. However, depth resolution typically decreases when focusing below the surface of a sample, which limits the applicability. A computational model is introduced that calculates the axial resolution, its decay, and the attenuation coefficient from confocal through-plane scans of translucent layers. The model is benchmarked with different polymers and objectives (air, water, oil) using a confocal Raman microscope. The algorithm requires a single through-plane scan that allows to identify the sample by signal intensity differences. It fits the point spread function of the objective at the top and bottom interface of the specimen to extract the re

SUBMITTER: Maier M 

PROVIDER: S-EPMC11935062 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature