Multiplexed Single-Cell Rheology Probing Using Surface Acoustic Waves.

ABSTRACT: Cellular rheological properties affect cell function and are reflective of cell status. It is challenging to perform multiplexed single-cell rheology probing with high controllability, particularly for adherent cells. A surface acoustic wave (SAW)-based method is presented for this purpose. The method integrates the potent micromanipulation ability of acoustic waves in a microfluidic chamber with the ability of cell-anchored microbeads to concentrate the acoustic energy to deform the cell. Two strategies are developed for placing a targeted microbead at a desired position on the cell membrane. The power-law rheological dynamics with plastic components are applied to fit the creep (during the mechanical loading) and relaxation (after force removal) responses of the cell. With more than 400

SUBMITTER: Hu Y 

PROVIDER: S-EPMC11935143 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature