Optimal Biofunctionalization of Gold Nanoislands for Electrochemical Detection of Soluble Programmed Death Ligand 1.

ABSTRACT: Soluble programmed death ligand-1 (sPD-L1), a pivotal immune checkpoint protein, serves as a biomarker for evaluating the efficacy of cancer therapies. Aptamers, as highly stable and specific recognition elements, play an essential role in emerging point-of-care diagnostic technologies. Yet, crucial advancements rely on engineering the intricate interaction between aptamers and sensor substrates to achieve specificity and signal enhancement. Here, a comprehensive physicochemical characterization and performance optimization of a sPD-L1 aptamer-based biosensor by a complementary set of state-of-the-art methodologies is presented, including atomic force microscopy-based infrared spectroscopy and high-resolution transmission electron microscopy, providing critical insights on the surface cove

SUBMITTER: Lotfibakalani Z 

PROVIDER: S-EPMC11935212 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature