Double-Orthogonal Gradient-Based High-Throughput Screening Platform for Studying Cell Response Toward Combined Physicochemical Biomaterial Properties.

ABSTRACT: Implant-associated complications arise due to non-optimized cell-biomaterial interactions. It is well known that cells respond to their physicochemical microenvironment on 2D interfaces and 3D networks. Attempts to manipulate this interaction target surface parameters such as wettability (W), stiffness (S), and topography (T) to influence cell differentiation, adhesion, and morphology, due to induction of gene activation and protein expression. Investigating the combinatorial influence of all three mentioned parameters simultaneously remains challenging, though most realistic, since all three parameters are inherently present on a surface. Herein, a novel high-throughput screening technology, which allows investigating the cell response of human bone-marrow-derived mesenchymal stem cells t

SUBMITTER: van der Boon TAB 

PROVIDER: S-EPMC11935256 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature