Binding of Perfluoroalkyl Substances to Nanoplastic Protein Corona Is pH-Dependent and Attenuates Their Bioavailability and Toxicity.

ABSTRACT: There is a severe lack of understanding of the effects of micro/nanoplastics on human proteins and cells, especially in the presence of organic pollutants. Herein, both in vivo and in vitro assays are conducted to structurally evaluate blood protein complexed with perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) as well as their coronas formed on polystyrene nanoplastics (PNs). PFOS is bound to serum albumin (SA) about 4 times as firmly as PFOA, which is not influenced by protein corona formation onto PN surfaces. However, the small molecular binding dramatically suppresses SA-PN aggregation. Low pH weakens the protein interaction of PFOS while not PFOA, which is also independent of PN adsorption, but the interaction with SA is still stronger for PFOS than PFOA, indicatin

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC11935290 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature