Cervical heterotopic heart transplantation in mice using a novel suture technique.

ABSTRACT:

Background

Vascularized transplantation models in mice are critical to understand mechanisms that mediate rejection and to develop new therapeutics. Standard abdominal heterotopic heart transplantation techniques employ an end-to-side suture technique and are the workhouse of transplant immunology research laboratories. Recently, cervical heterotopic heart transplantation in mice has emerged as an alternative due to several advantages but is conventionally performed by suture or cuff techniques in an end-to-end fashion. Therefore, we introduce an end-to-side anastomosis technique.

Methods

The donor pulmonary artery is end-to-side anastomosed to the recipient right external jugular vein, using a continuous 10-0 nylon suture. Vascular suturing is ac

SUBMITTER: Dun H 

PROVIDER: S-EPMC11935459 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature