Synthetic Antiangiogenic Vascular Endothelial Growth Factor-A Splice Variant Revascularizes Ischemic Muscle in Peripheral Artery Disease.

ABSTRACT:

Background

Alternative splicing in the eighth exon C-terminus of VEGF-A (vascular endothelial growth factor-A) results in the formation of proangiogenic VEGF₁₆₅a and antiangiogenic VEGF₁₆₅b isoforms. The only known difference between these 2 isoform families is a 6-amino acid switch from CDKPRR (in VEGF₁₆₅a) to SLTRKD (in VEGF₁₆₅b). We have recently shown that VEGF₁₆₅b can induce VEGFR2-activation but fails to induce VEGFR1 (VEGF receptor 1)-activation. The molecular mechanisms that regulate VEGF₁₆₅b's ability toward differential VEGFR2 versus VEGFR1 activation/inhibition are not yet clear.

Methods and results

Hypoxia serum starvation was used as an in vitro peripheral artery disease model. Unilateral single l

SUBMITTER: Raja A 

PROVIDER: S-EPMC11935576 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature