The natural variation allele OsGSW3.2 in Oryza rufipogon is involved in brassinosteroid signaling and influences grain size and weight.

ABSTRACT: Oryza rufipogon is the ancestor of cultivated rice and harbors many elite genes; thus, this plant is an important germplasm for improving rice varieties. Grain size is an important factor in determining rice yield and quality. In this study, we identified a natural variation allele from the O. rufipogon inbred line Huaye3 (HY3), which is located on chromosome 3 and named it GRAIN SIZE and WEIGHT 3.2 (OsGSW3.2). The OsGSW3.2 knockout (KO) mutant presented increased grain size and weight, which was associated with decreased chlorophyll content and long awns. The overexpression of OsGSW3.2^HY3 caused a significant decrease in grain size and weight. OsGSW3.2 negatively regulates grain size through cell proliferation. Transcriptomic analysis of spikelet hulls from the KO lines and wil

SUBMITTER: Liang X 

PROVIDER: S-EPMC11936100 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature