Bacterial lysis or survival after infection with phage Sf14 depends on combined nutrient and temperature conditions.

ABSTRACT: Bacteriophage Sf14 is a Moogle-like myovirus that infects Shigella flexneri. S. flexneri is a human pathogen that replicates intracellularly in the intestine, but it persists in a low metabolic state in environmental fresh water sources. Though closely related to FelixO1, Moogleviruses were more recently discovered within the last 10 years; thus, mechanistic knowledge of their infection cycles is still being gathered. This work investigated the combined effects of temperature and nutrient concentration on both host growth and phage replication. In combination, a total of 16 different conditions were analyzed. Results indicate that nutrient-rich media facilitate shorter infection cycles and support phage production at all temperatures. As nutrient content decreased, temperature significantl

SUBMITTER: Ross ND 

PROVIDER: S-EPMC11936213 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature