Characterizing serotonin expression throughout bovine mammary gland developmental stages and its relationship with 17β-estradiol at puberty.

ABSTRACT: Serotonin acts in an autocrine/paracrine manner within the mammary epithelium regulating cell homeostasis during lactation and cell turnover during involution after milk stasis. However, the presence and role of mammary serotonin during the pubertal developmental stage is unknown in the bovine. Here, we characterized the serotonin receptor profile and serotonin immunolocalization in bovine mammary tissue at eight developmental stages (i.e., birth, weaning, puberty, six months gestation, early lactation, mid-lactation, early dry and late dry, n = 6/stage). Further, we investigated the effects of 5-HTP (serotonin precursor), 17β-estradiol (E2), and ICI 182780 (ERα antagonist) either alone or in various combinations (i.e., 5-HTP +  E2, 5-HTP + ICI, E2 + ICI or 5-HTP + E2 +  ICI) on cultured b

SUBMITTER: Field SL 

PROVIDER: S-EPMC11936267 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature