Whole-brain mapping in adult zebrafish and identification of the functional brain network underlying the novel tank test.

ABSTRACT: Zebrafish have gained prominence as a model organism in neuroscience over the past several decades, generating key insight into the development and functioning of the vertebrate brain. However, techniques for whole brain mapping in adult stage zebrafish are lacking. Here, we describe a pipeline built using open-source tools for whole-brain activity mapping in adult zebrafish. Our pipeline combines advances in histology, microscopy, and machine learning to capture cfos activity across the entirety of the brain. Following tissue clearing, whole brain images are captured using light-sheet microscopy and registered to the recently created adult zebrafish brain atlas (AZBA) for automated segmentation. By way of example, we used our pipeline to measure brain activity after zebrafish were

SUBMITTER: Rajput N 

PROVIDER: S-EPMC11936448 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature