TRPV4 activation in Schwann cells mediates mechanically induced pain of oral cancer.

ABSTRACT:

Introduction

Patients with oral cancer often experience intense functional pain due to mechanical stimulation at the cancer site. The role of mechanosensitive ion channels in oral cancer pain, such as TRPV4, is not fully understood.

Objectives

Our objective was to investigate the role of Schwann cell TRPV4 in oral cancer pain.

Methods

We examined the impact of TRPV4 inhibition on oral cancer pain in NU/J and C57BL/6J mice injected with human tongue cancer cell line (HSC-3) and mouse oral cancer cell line (MOC2) in the hind paw or tongue. Mechanical and heat sensitivity were assessed using the von Frey and Hargreaves tests, respectively. TRPV4 expression and functional activity in Schwann cells were analyzed using immunohistochemistry, qRT-PCR, Ca²⁺ imaging,

SUBMITTER: Mulpuri Y 

PROVIDER: S-EPMC11937083 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature