Super-resolution optical microscopy reveals accumulation of photoactivable dengue protein (Dendra2-NS2B) in the endoplasmic reticulum.

ABSTRACT: The non-structural protein NS2B plays a critical role in the maturation of Dengue virus (DENV Type 2). The underlying mechanism and the role of NS2B are largely unknown due to the unavailability of its location of activity in the target organelle at single molecule level. This is largely due to the incapability of existing optical microscopes to resolve features beyond the diffraction limit of light ([Formula: see text]), which is limited to a few hundred nanometers. Existing microscopy techniques are at best useful for ensemble study and details at the single molecule level remain hidden. To enable single-molecule resolution, we investigated the role of NS2B protein in a cellular system using scanning single molecule localization microscopy (scanSMLM). Accordingly, a photoactivable plasmi

SUBMITTER: Aravinth S 

PROVIDER: S-EPMC11937263 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature