Super-Resolution Goes Viral: T4 Virus Particles as Versatile 3D-Bio-NanoRulers.

ABSTRACT: In the burgeoning field of super-resolution fluorescence microscopy, significant efforts are being dedicated to expanding its applications into the 3D domain. Various methodologies have been developed that enable isotropic resolution at the nanometer scale, facilitating the visualization of 3D subcellular structures with unprecedented clarity. Central to this progress is the need for reliable 3D structures that are biologically compatible for validating resolution capabilities. Choosing the optimal standard poses a considerable challenge, necessitating, among other attributes, precisely defined geometry and the capability for specific labeling at sub-diffraction-limit distances. In this context, the use of the non-human-infecting virus, bacteriophage T4 is introduced as an effective and st

SUBMITTER: Gallea JI 

PROVIDER: S-EPMC11937993 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature