Redesigned Pathway for <i>De Novo</i> Synthesis of Vanillin and Co-conversion of Multiple Renewable Substrates in <i>Saccharomyces cerevisiae</i>.

ABSTRACT: Vanillin, known as the "queen of flavors", is an extensively used and important aromatic compound with multiple functions, including aldehyde, ether, and hydroxyl. Converting lignocellulosic biomass-derived substrates to natural vanillin is sustainable and economical through artificial cell factories. However, the inefficiency of exogenous enzymes and the toxic effect of vanillin on cells limit its production. In this study, pathway reconstruction and integration site optimization of Saccharomyces cerevisiae enabled the production of 363.0 mg/L of vanillin in just four steps, with chorismate serving as an intermediate, by enhancing the endogenous shikimate pathway. A feeding strategy further yielded a record titer of 533.0 mg/L vanillin using engineered S. cerevisiae in flask

SUBMITTER: Xin X 

PROVIDER: S-EPMC11937998 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature