Effects of high light exposure and heterologous expression of β-carotene ketolase on the metabolism of carotenoids in <i>Chlamydomonas reinhardtii</i>.

ABSTRACT:

Introduction

Stress from high light exposure and overexpression of β-carotene ketolase can have significant effects on the synthesis of carotenoids in Chlamydomonas reinhardtii. As a promising platform for carotenoid production, C. reinhardtii needs further research and technological innovation to address challenges, such as environmental interference, exogenous gene expression, and metabolic regulation, to achieve efficient and sustainable production of carotenoids.

Methods

Appropriate β-carotene ketolase were selected from different organisms and subjected for codon optimization based on the preferences of the nuclear genome of C. reinhardtii. After designation, including intron insertion and chloroplast transit peptide, expression vectors were constru

SUBMITTER: Mei R 

PROVIDER: S-EPMC11938120 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature