Genomic analysis of extended-spectrum beta-lactamase-producing <i>E. coli</i> from Czech diary calves and their caretakers.

ABSTRACT:

Introduction

The increasing prevalence of antimicrobial resistance in livestock, particularly the dissemination of extended-spectrum beta-lactamase-producing Escherichia coli, poses a significant zoonotic and public health risk. This study investigates the genomic characteristics of cefotaxime-resistant E. coli isolates from dairy calves across 23 Czech farms and their caretakers.

Materials and methods

Bacteriological cultivation on McConkey agar with cefotaxime was used for their isolation, susceptibility to selected antibiotics was determined by disc diffusion method, production of extended-spectrum beta-lactamases (ESBL) was demonstrated by double disc synergy test. The PCR was applied to confirm the presence of selected genes encoding resistance to some bet

SUBMITTER: Masarikova M 

PROVIDER: S-EPMC11938843 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature