Single-cell RNA-seq reveals that granulosa cells are a target of phthalate toxicity in the ovary.

ABSTRACT: Phthalates are known endocrine-disrupting chemicals and ovarian toxicants that are used widely in consumer products. Phthalates have been shown to exert ovarian toxicity on multiple endpoints, altering transcription of genes responsible for normal ovarian function. However, the molecular mechanisms by which phthalates act on the ovary are not well understood. In this study, we hypothesized that phthalates specifically target granulosa cells within the ovarian follicle. To test our hypothesis, we cultured whole mouse antral follicles for 96 h in the presence of vehicle or 10 µg/ml of a phthalate metabolite mixture. At the end of the culture period, follicles were dissociated into single-cell suspensions and subjected to single-cell RNA-sequencing. We used markers from published studies to i

SUBMITTER: Mattson E 

PROVIDER: S-EPMC11939077 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature