Development of a Monoclonal Antibody Against Duck IFN-γ Protein and the Application for Intracellular Cytokine Staining.

ABSTRACT: Interferon-γ (IFN-γ), a member of the Type II IFN family, is a crucial cytokine in the immune system and serves as an important indicator of immune response. Intracellular cytokine staining (ICS) is a technique used to analyze the production of cytokines within individual cells, and it has a wide range of applications in the fields of immunological monitoring, vaccine trials, and the study of infectious diseases. This study aimed to prepare monoclonal antibodies against duck IFN-γ protein and to establish an ICS protocol for detecting the duck IFN-γ protein. The duIFN-γ-His or duIFN-γ-Fc gene was cloned into the pEE12.4 expression vector and expressed as a recombinant protein of size 20.2 KDa or 54.9 KDa in 293F cells. The purified recombinant proteins were inoculated into BA

SUBMITTER: Chen Y 

PROVIDER: S-EPMC11939334 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature