Development of a Low-Cost and Easy-Assembly Capillary Electrophoresis System for Separation of DNA.

ABSTRACT: Capillary electrophoresis based on laser-induced fluorescence (CE-LIF) plays an important role in the analysis of nucleic acids. However, the commercial CE-LIF is not only quite expensive but also inflexible, thus hindering its widespread use in the lab. Herein, we proposed a compact, low-cost, and flexible CE-LIF system. We also investigated its stability by separating the DNA ladders. Experiments demonstrated that the relative standard error of the relative fluorescence intensity and migration time was lower than 6.2% and 1.1%, respectively. The aperture size of the light source illuminating the capillary can affect the separation performance. Smaller apertures offer higher resolution length for the adjacent DNA fragments but may reduce the number of theoretical plates. Various fluoresce

SUBMITTER: Li J 

PROVIDER: S-EPMC11939447 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature