Comprehensive Phenotypic Characterization and Genomic Analysis Unveil the Probiotic Potential of <i>Bacillus velezensis</i> K12.

ABSTRACT: Bacillus spp. have emerged as pivotal sources of probiotic preparations, garnering considerable attention in recent years owing to their vigorous bacteriostatic activity and antimicrobial resistance. This study aimed to investigate these probiotic characteristics in depth and verify the safety of Bacillus velezensis K12, a strain isolated from broiler intestine. The K12 strain was identified as Bacillus velezensis based on its morphology and 16S rDNA sequence homology analysis. Subsequently, B. velezensis K12 was evaluated for acid resistance, bile salt resistance, gastrointestinal tolerance, drug sensitivity, and antimicrobial activity. Additionally, whole-genome sequencing technology was employed to dissect its genomic components further, aiming to explore its

SUBMITTER: Tang Y 

PROVIDER: S-EPMC11939506 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature