Establishment and Characterization of OFT and OFO Cell Lines from Olive Flounder (<i>Paralichthys olivaceus</i>) for Use as Feeder Cells.

ABSTRACT: Olive flounder (Paralichthys olivaceus) is a commercially important fish species in Japan, China, and the Republic of Korea. Despite numerous attempts to improve productivity, there have been no studies of in vitro germline stem cell (GSC) culture in this species. Here, olive flounder testicular and ovarian cell lines (OFT and OFO, respectively) were established and characterized. RT-PCR demonstrated that OFT and OFO expressed several gonadal somatic cell markers, including wt1 and fgf2, but lacked expression of germ cell markers, such as vasa, nanos2, and scp3. In addition, SNP analysis revealed that OFT originated from XY male P. olivaceus and OFO originated from XX female P. olivaceus. These results suggest that OFT was composed of

SUBMITTER: Jo JY 

PROVIDER: S-EPMC11939788 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature