Untargeted Metabolomic Profiling of Colonic Mucosa in Individuals with Irritable Bowel Syndrome.

ABSTRACT: Background: Irritable Bowel Syndrome (IBS) is a complex disorder characterized by altered gut-brain interactions, with gastrointestinal microbiota and metabolic dysregulation playing key roles in its pathophysiology. Identifying specific metabolic alterations within the colonic mucosa may enhance our understanding of IBS and contribute to improved diagnostic and therapeutic approaches. Methods: This cross-sectional study analyzed the metabolomic profiles of colonic mucosal biopsies from 44 IBS patients assessed with ROME IV criteria and 69 healthy controls undergoing colonoscopy. Untargeted metabolomic profiling was conducted using liquid chromatography-mass spectrometry (LC-MS), and differential metabolite analysis was performed via fold-change calculations and machine learn

SUBMITTER: Krynicka P 

PROVIDER: S-EPMC11940239 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature