Multi-Channel Cellytics for Rapid and Cost-Effective Monitoring of Leukocyte Activation.

ABSTRACT: Morphological changes in leukocytes are valuable markers for diseases and immune responses. In our earlier work, we presented Cellytics, a device that uses lens-free shadow imaging technology (LSIT) to monitor natural killer cell activity. Here, we present an improved Cellytics system that has been upgraded to a four-channel configuration to achieve higher throughput while maintaining robust reproducibility for rapid and cost-effective leukocyte analysis. The performance of this multi-channel Cellytics system was improved through refinements to the micro-pinhole chip. Etched pinholes provided better image resolution and clarity compared to drilled pinholes. To stimulate leukocytes, we used an activation stimulator cocktail (ASC) and quantified the resulting morphological changes using shad

SUBMITTER: Cheon H 

PROVIDER: S-EPMC11940678 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature