Novel Roles of Nestin in Postnatal Root Formation.

ABSTRACT: Background/Objectives: Nestin is an intermediate filament protein and a marker of odontoblasts, but its function in tooth and bone formation is largely unknown. This study aimed to determine whether Nestin plays a role in postnatal tooth formation. Methods: 4-week-old Nestin knockout (KO) mice were analyzed with a range of techniques, including X-ray imaging, uCT, backscattered and acid-etched casted SEM, FITC-confocal microscopy, H&E and TRAP staining, and immunohistochemistry. Results: The KO mice had no apparent difference in crown formation compared to age-matched wild-type (WT) but showed delayed molar eruption with reduced TRAP⁺ osteoclasts. More importantly, KO mice developed expanded predentin and shorter, thinner roots with irregular and shortened d

SUBMITTER: Jing Y 

PROVIDER: S-EPMC11941000 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature