Reactions of <i>Plasmodium falciparum</i> Type II NADH: Ubiquinone Oxidoreductase with Nonphysiological Quinoidal and Nitroaromatic Oxidants.

ABSTRACT: In order to detail the antiplasmodial effects of quinones (Q) and nitroaromatic compounds (ArNO₂), we investigated their reduction mechanism by Plasmodium falciparum flavoenzyme type II NADH:ubiquinone oxidoreductase (PfNDH2). The reactivity of Q and ArNO₂ (n = 29) follows a common trend and exhibits a parabolic dependence on their single-electron reduction potential (E71), albeit with significantly scattered data. The reactivity of quinones with similar E71 values increases with their lipophilicity. Quinones are reduced by PfNDH2 in a two-electron way, but ArNO₂ are reduced in a single-electron way. The inhibition studies using NAD⁺ and ADP-ribose showed that quinones oxidize the complexes of reduced enzyme with NADH

SUBMITTER: Miseviciene L 

PROVIDER: S-EPMC11941790 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature