A Novel Peptide-Based Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Neutralizing Antibodies Against NADC30-like PRRSV GP5 Protein.

ABSTRACT: Porcine reproductive and respiratory syndrome (PRRS) is a pig respiratory disease threating the global swine industry. To combat PRRS, it is necessary of the effective diagnostic detection of antibody, including developing a neutralizing antibody against porcine reproductive and respiratory syndrome virus (PRRSV), especially the currently prevalent NADC30-like PRRSV in China. In this study, we prepared three monoclonal antibodies (mAbs) against NADC30-like PRRSV glycoprotein 5 (GP5) protein, and identified two corresponding precise epitopes (¹⁵⁵WR¹⁵⁶ and ¹⁹⁶QWGRP²⁰⁰). In the neutralization test, ¹⁹⁶QWGRP²⁰⁰ recognizing GP5 mAbs (11E6 and 12D1) exhibited obvious neutralizing activity, whereas the ¹⁵⁵WR¹⁵⁶

SUBMITTER: Sun S 

PROVIDER: S-EPMC11941917 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature