Reported transgenerational responses to <i>Pseudomonas aeruginosa</i> in <i>Caenorhabditis elegans</i> are not robust.

ABSTRACT: We report our attempt to replicate reports of transgenerational epigenetic inheritance in Caenorhabditis elegans. Multiple laboratories report that C. elegans adults and their F1 embryos exposed to the pathogen Pseudomonas aeruginosa show pathogen aversion behavior and increased daf-7/TGFβ reporter gene expression. However, results from one group show persistence of both through the F4 generation. We failed to consistently detect either the avoidance response or elevated daf-7 expression beyond the F1 generation. We confirmed that the dsRNA transport proteins SID-1 and SID-2 are required for intergenerational (F1) inheritance of pathogen avoidance, but not for the F1 inheritance of elevated daf-7 expression. Reanalysis of RNA seq data provides addi

SUBMITTER: Gainey DP 

PROVIDER: S-EPMC11942167 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature