In Silico Characterization and Determination of Gene Expression Levels Under Saline Stress Conditions in the Zinc Finger Family of the C1-2i Subclass in <i>Chenopodium quinoa</i> Willd.

ABSTRACT: Quinoa (Chenopodium quinoa) is recognized for its tolerance to abiotic stress, including salinity, and its recent genome sequencing has facilitated the study of the mechanisms underlying this adaptation. This study focused on characterizing the ZAT genes of the C2H2 subfamily in quinoa, evaluating their expression under saline stress. Eight ZAT genes were identified and analyzed in silico using genomic databases and bioinformatics tools, assessing their conserved domains, cis-regulatory motifs, and physicochemical characteristics. Additionally, germination assays, hydroponic cultivation, and gene expression analyses via qPCR were performed on halotolerant (UNSA_VP033) and halosensitive (UNSA_VP021) accessions exposed to different NaCl concentrations. The genes CqZAT4 and C

SUBMITTER: Alvarez-Vasquez A 

PROVIDER: S-EPMC11942331 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature