The PKS-NRPS Gene <i>BBA_09856</i> Deletion Mutant of <i>Beauveria bassiana</i> Enhanced Its Virulence Against <i>Ostrinia furnacalis</i> Larvae and Strengthened the Host Plant's Resistance to <i>Botrytis cinerea</i> as an Endotype.

ABSTRACT: Nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) play crucial roles in the development and pathogenicity of the entomopathogenic fungus Beauveria bassiana. However, they are among the few biosynthetic gene clusters with unknown functions in B. bassiana. To investigate the role of the hybrid PKS-NRPS synthetase gene BBA_09856 in B. bassiana, we constructed a mutant strain, ∆BBA09856-WT, by deleting the BBA_09856 gene through Agrobacterium-mediated transformation. We then analyzed the biological characteristics of the mutant strain and the virulence of the mutant strain toward Ostrinia furnacalis larvae, as well as its antagonistic effects against the phytopathogen Botrytis cinerea. We found that the average growth r

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11942696 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature