Whole-Genome Sequencing of <i>Flammulina filiformis</i> and Multi-Omics Analysis in Response to Low Temperature.

ABSTRACT: The growth of Flammulina filiformis is strongly dependent on low-temperature cues for the initiation of primordia formation. To obtain a comprehensive understanding of the molecular mechanisms that govern the mycelial response to cold stress, de novo genome sequencing of the F. filiformis monokaryon and multi-omics data (transcriptome and metabolome) analyses of the mycelia, primordia, and fruiting bodies were conducted in the present study. Genome sequencing based on PacBio HiFi and Hi-C resulted in a 36.3 Mb genome sequence that mapped to 12 chromosomes, comprising 11,886 protein-coding genes. A total of 25 cold-responsive (COR) genes and 520 cold-adapted enzymes were identified in the genome. Multi-omics analyses showed that the pathways related to carbohydrate metabolism

SUBMITTER: Liang X 

PROVIDER: S-EPMC11942922 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature