Effects of Feeding Sources and Different Temperature Changes on the Gut Microbiome Structure of <i>Chrysomya megacephala</i> (Diptera: <i>Calliphoridae</i>).

ABSTRACT: Chrysomya megacephala (Diptera: Calliphoridae), commonly referred to as the oriental latrine fly, is a synanthropic blowfly species frequently associated with decomposing organic matter. This study sought to investigate the influence of various feeding substrates and temperature conditions, specifically constant temperatures of 15, 25, 35 °C, and variable temperatures averaging 23.31 °C, on the gut microbiome of C. megacephala. The microbiome analysis was conducted using the Illumina HiSeq platform for 16S rRNA gene sequencing in Changsha, China. Across all experimental conditions, the gut microbiome of C. megacephala yielded 1257 operational taxonomic units (OTUs), which were categorized into 26 phyla, 72 classes, 165 orders, 270 families, 516 genera, and 794 s

SUBMITTER: Ngando FJ 

PROVIDER: S-EPMC11943086 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature