The Interaction Between the <i>asb5a</i> and <i>asb5b</i> Subtypes Jointly Regulates the L-R Asymmetrical Development of the Heart in <i>Zebrafish</i>.

ABSTRACT: The asb5 gene, a member of the Asb protein subfamily characterized by six ankyrin repeat domains, is highly conserved and comprises two subtypes, asb5a and asb5b, in zebrafish. Our previous research has demonstrated that a deficiency of the asb5 gene significantly impairs early cardiac contractile function, highlighting its close relationship with heart development. Zebrafish asb5 expression was disrupted by both morpholino (MO) antisense oligomer-mediated knockdown and a CRISPR-Cas9 system. A high-throughput RNA-Seq analysis was used to analyze the possible molecular regulatory mechanism of asb5 gene deletion leading to left-right (L-R) asymmetry defects in the heart. Whole-mount in situ hybridization (WISH) was conducted to evaluate gene e

SUBMITTER: Zhou W 

PROVIDER: S-EPMC11943173 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature