Construction of a Plasmid-Free <i>Escherichia coli</i> Strain with Enhanced Heme Supply to Produce Active Hemoglobins.

ABSTRACT:

Background

Heme is an important cofactor and plays crucial roles in the correct folding of hemoproteins. The synthesis of heme can be enhanced by the plasmid-based expression of heme biosynthetic genes. However, plasmid-based expression is genetically unstable and requires the utilization of antibiotics to maintain high copy numbers of plasmids.

Methods

The rate-limiting steps in heme biosynthesis were first analyzed based on previous studies and the accumulation of heme intermediates was achieved by adding heme precursor (5-aminolevulinic acid, ALA). Next, the intracellular accumulation of porphyrin was increased by deleting the porphyrin transporter TolC. Finally, the heme synthetic genes were modified by integrating the hemA and hemL genes into the cheW</

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC11943725 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature