Characterization of Flexusin A, a Novel Circular Bacteriocin Produced by Marine Bacterium <i>Bacillus flexus</i> R29-2.

ABSTRACT: Circular bacteriocins are potent antimicrobials against pathogenic Gram-positives. In searching for marine bacteriocins, an antibacterial peptide (flexusin A) was purified from the fermentation broth of marine bacterium Bacillus flexus R29-2. Genome sequencing and gene annotation revealed the chromosome contained an unknown circular bacteriocin gene cluster. Approaches including shot-gun proteomics analysis, AntiSMASH and BAGEL4 predication as well as the comprehensive sequence alignment, were then conducted, respectively, to verify the correlation of flexusin A with the gene-encoded precursor peptide. The results confirmed that flexusin A was the mature circular bacteriocin of the predicated precursor peptide with six amino acids as leader peptide. Flexusin A was 6098.4 Da in size,

SUBMITTER: Qiao X 

PROVIDER: S-EPMC11943950 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature