Fractionation of a Procyanidin-Rich Grape Seed Extract by a Preparative Integrated Ultrafiltration/Reverse Osmosis/Solid-Phase Extraction Procedure.

ABSTRACT: The consumption of grape seed extracts is known for its contribution to animal and human health and is associated with its relevant procyanidin content. However, there is a little scientific unanimity whether these properties are due to the procyanidin content or to the length of their polymers. The main reason for this doubt is the technical difficulties related to their separation. Therefore, a preparative separation of grape seed extract was carried out using an integrated ultra/diafiltration procedure with membranes of 300, 30, 5, and 1 kDa molecular mass cut-offs, reverse osmosis and solid-phase extraction to obtain fractions of very high (>300 kDa), high (300-30 kDa), intermediate (30-5 kDa), low molecular mass (5-1 kDa), very-low-mass polar molecules and ions (<1 kDa), and very-low-

SUBMITTER: Guerrero-Hurtado E 

PROVIDER: S-EPMC11944240 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature