Network Pharmacology-Based Elucidation of the Hypoglycemic Mechanism of <i>Grifola frondosa</i> GF5000 Polysaccharides via GCK modulation in Diabetic Rats.

ABSTRACT:

Background/objectives

Our lab has previously reported that Grifola frondosa (maitake mushroom) GF5000 has antidiabetic potential owing to its ability to improve insulin resistance. This study aimed to gain insight into the system-level hypoglycemic mechanisms of GF5000 using transcriptomics, proteomics, and network pharmacology. This study provides new insights into the hypoglycemic mechanisms of GF5000, identifying key molecular targets involved in mitigating insulin resistance in T2DM.

Methods

Liver protein and gene expression in normal control (NC), diabetic control (DC), and GF5000-treated (GF5000) rats were analyzed via iTRAQ and RNA-seq. The relationships between differentially expressed genes (DEGs), differentially expressed proteins (DEPs), and type 2 diabetes

SUBMITTER: Xiao C 

PROVIDER: S-EPMC11944433 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature