Whole-Genome Sequencing of Linezolid-Resistant and Linezolid-Intermediate-Susceptibility <i>Enterococcus faecalis</i> Clinical Isolates in a Mexican Tertiary Care University Hospital.

ABSTRACT: Linezolid-non-susceptible Enterococcus faecalis (LNSEf) has emerged as a critical clinical concern worldwide, yet data from Latin American settings remain scarce. This study aimed to investigate the molecular epidemiology and mechanisms underlying LNSEf in a Mexican tertiary care university hospital, focusing on clinical correlates and clonal relationships. A total of 392 non-duplicated E. faecalis isolates were collected over 12 months, of which 24 with minimum inhibitory concentrations ≥4 µg/mL underwent whole-genome sequencing to identify specific resistance determinants (optrA, cfrA, 23S rRNA mutations) and to perform multilocus sequence typing (MLST) and phylogenetic analyses. Of the 392 isolates, 6.12% showed linezolid non-susceptibility, predominan

SUBMITTER: Martinez-Ayala P 

PROVIDER: S-EPMC11944505 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature