Identification and Characterization of Copper-Responsive miRNAs and Their Target Genes in Jerusalem Artichoke.

ABSTRACT: microRNAs (miRNAs) are key regulators of gene expression in plants, significantly contributing to various biological processes and stress responses. While their roles have been extensively studied in Arabidopsis thaliana and other model plants, the response of miRNAs to copper (Cu) stress in Jerusalem artichoke remains unknown. This study addresses this gap by investigating Cu-responsive miRNAs and their regulatory roles in Jerusalem artichoke under Cu stress. Through small RNA library analysis, six miRNA families-miR168, miR394, miR397, miR398, miR408, and miR858-were identified in Cu-stressed and control plants of the Jerusalem artichoke cv. NY1. These miRNAs possess characteristic stem-loop precursor structures and detectable miRNA* sequences, with miR858 having unusually long pr

SUBMITTER: Chen X 

PROVIDER: S-EPMC11945104 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature