Molecular Detection of <i>bla</i><sub>TEM</sub> and <i>bla</i><sub>SHV</sub> Genes in ESBL-Producing <i>Acinetobacter baumannii</i> Isolated from Antarctic Soil.
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ABSTRACT: The phenomenon of antimicrobial resistance (AMR) in cold environments, exemplified by the Antarctic, calls into question the assumption that pristine ecosystems lack clinically significant resistance genes. This study examines the molecular basis of AMR in Acinetobacter spp. Isolated from Antarctic soil, focusing on the blaTEM and blaSHV genes associated with extended-spectrum beta-lactamase (ESBL) production; Soil samples were collected and processed to isolate Antarctic soil bacteria. Molecular detection was then conducted using polymerase chain reaction (PCR) to identify the bacteria species by 16S rRNA/rpoB and 10 different beta-lactamase-producing genes. PCR amplicons were sequenced to confirm gene identity and analyze genetic variabil
SUBMITTER: Pazos C
PROVIDER: S-EPMC11945639 | biostudies-literature | 2025 Feb
REPOSITORIES: biostudies-literature
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