Huperzine A Production and Acetylcholinesterase Inhibition by <i>Phlegmariurus taxifolius</i> Cell Suspension Culture: A Comparative Study in Flasks and an Airlift Bioreactor.

ABSTRACT: Background: The callus cultures from the fronds of the lycophyte Phlegmariurus taxifolius produce the huperzine A (HupA) alkaloid, which is used in Alzheimer's disease treatment. This study aimed to establish the growth kinetics and HupA production by the newly HupS21 cell line grown in 250 mL flasks and in a 2 L airlift bioreactor. Methods: Batch-type kinetics were carried out for 60 days in 250 mL flasks and for 20 days in a 2 L airlift bioreactor. Measurements of dry weight (DW), specific growth rate (μ), doubling time (dt), pH, carbohydrate consumption, and HupA quantification were performed. The acetylcholinesterase (AChE) inhibitory assay of the HupS21 alkaloidal extract was determined. Results: The 250 mL flasks kinetic reached a maximum cell growth of 8.

SUBMITTER: Perez Aguilar RDC 

PROVIDER: S-EPMC11946177 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature