Extraction of Cannabinoids and Terpenes from Hemp Flowers and Leaves (<i>Cannabis sativa</i> L., Futura 75): Chemical Profiling and Evaluation of Anticancer Properties.

ABSTRACT: This study investigated efficient extraction methods for cannabinoids and terpenes from the above-ground parts of Futura 75, focusing on two techniques: pressurized extraction and magnetic stirrer-assisted extraction. The effects of solvent type, temperature, time, and pressure were evaluated using five organic solvents and two binary solvent systems. Cannabinoid profiles of obtained extracts were analyzed using gas chromatography coupled with mass spectrometry (GC-MS), while terpene profiles were characterized through solid-phase microextraction (SPME) combined with GC-MS. Next, two selected extracts with the highest content of cannabinoid and terpene fractions (Futu1 and Futu2) were tested for antiproliferative activity toward cancer cell lines (MV4-11, AGS, HT-29, MDA-MB-468, MCF-7) and

SUBMITTER: Haczkiewicz M 

PROVIDER: S-EPMC11946255 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature